2013 Fiscal Year Final Research Report

The development of polysaccharide-encapsulated liposomes as vaccines against fungal infections

Research Project

Project/Area Number	24890255
Research Category	Grant-in-Aid for Research Activity Start-up
Allocation Type	Single-year Grants
Research Field	Bacteriology (including Mycology)
Research Institution	Tokyo University of Pharmacy and Life Science
Principal Investigator	TADA RUI 東京薬科大学, 薬学部, 助教 (70635888)
Project Period (FY)	2012-08-31 – 2014-03-31
Keywords	リポソーム / 多糖 / 粘膜免疫 / 全身免疫 / 真菌症 / 粘膜ワクチン / 粘膜アジュバント
Research Abstract	Our laboratory has long been working on the liposomes with respect to immunological approaches. In the course of our study, we have already found that a nasal administration of the cationic liposomes with protein antigens dramatically enhanced both antigen-specific mucosal and systemic immune responses, namely the cationic liposome acts as a potent mucosal adjuvant in mice. In this study, we examined mucosal adjuvant activity of the cationic liposome against fungal polysaccharide antigens in order to overcome fungal infections. We first prepared polysaccharide antigens-encapsulated cationic liposomes, and then DBA/2 or BALB/c mice were immunized intranasally once a week with polysaccharide antigen-encapsulated cationic liposomes. Our study shows that intranasal vaccination with the polysaccharide antigen-encapsulated cationic liposomes significantly exerted polysaccharide antigen-specific antibody productions in DBA/2 mice, but not in BALB/c mice.

(4 results)