2013 Fiscal Year Final Research Report
Molecular Characterization of Glycosylation on Immunoglobulins: Implication for Pathogenesis of IgA Nephropathy
| Project/Area Number |
24890268
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Kidney internal medicine
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| Research Institution | Fujita Health University |
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Principal Investigator |
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| Project Period (FY) |
2012-08-31 – 2014-03-31
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| Keywords | IgA腎症 / 糖鎖異常IgA1 / 質量分析 / バイオマーカー探索 / 免疫グロブリン |
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| Research Abstract |
IgA1 with galactose (Gal)-deficient hinge-region O-glycans plays a key role in IgA nephropathy (IgAN). To characterize IgA1 involved with IgAN, O-glycan microheterogeneity and attachment sites should be analyzed. In this study, serum IgA1 from IgAN patients and healthy controls (HCs) was individually analyzed to define the glycosylation patterns and glycosylation sites using high-resolution mass spectrometry (MS). Although IgA1 from HCs contain some Gal-deficient O-glycans, the amount of Gal-deficient O-glycans in IgA1 from IgAN patients was greater than that in IgA1 O-glycans from HCs. Tandem MS revealed that the sites with Gal-deficient O-glycans included mainly three sites in IgA1 from IgAN patients as well as IgA1 from HCs. This study shows first definitive identification of O-glycosylation including sites attachment on IgA1 from IgAN patients. Our protocol provides useful information relevant to pathogenesis of IgAN through the identification of distinct O-glycosylation patterns.
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[Journal Article] Enzymatic Sialylation of IgA1 O-glycans : Implications for Studies of IgA Nephropathy2014
Author(s)
Takahashi K, Raska M, Stuchlova Horynova M, Hall SD, Poulsen K, Kilian M, Hiki Y, Yuzawa Y, Moldoveanu Z, Julian BA, Renfrow MB, Novak J
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Journal Title
PLoS ONE
Volume: (In-press)
Peer Reviewed
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[Journal Article] Comparison of effective impact among tonsillectomy alone, tonsillectomy combined with oral steroid and with steroid pulse therapy on long-term outcome of immunoglobulin A nephropathy2013
Author(s)
Yamamoto Y, Hiki Y, Nakai S, Yamamoto K, Takahashi K, Koide S, Murakami K, Tomita M, Hasegawa M, Kawashima S, Sugiyama S, Yuzawa Y
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Journal Title
Clin Exp Nephrol
Volume: 17
Pages: 218-24
Peer Reviewed
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[Presentation] IgA Antibodies to Glomerular Endothelial Cells in Patients with Lupus Nephritis : Correlations with Renal Injury2013
Author(s)
Takahashi K, Kondo A, Hirano D, Akiyama S, Hayashi H, Koide S, Hasegawa M, Yoshida S, Hiki Y, Miura K, Yuzawa Y
Organizer
46th Annual Meeting of the American Society of Nephrology
Place of Presentation
Atlanta, GA
Year and Date
20130000
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[Presentation] Enzymatic Sialylation of IgA1 Hinge-region : Pathogenic Role of Sialic Acid in IgA Nephropathy (IgAN)2013
Author(s)
Takahashi K, Raska M, Stuchlova Horynova M, Kasperova A, Hall S, Hiki Y, Yuzawa Y, Moldoveanu Z, Julian BA, Renfrow MB, Novak J
Organizer
46th Annual Meeting of the American Society of Nephrology
Place of Presentation
Atlanta, GA
Year and Date
20130000
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[Presentation] Synthesis of Galactose-deficient IgA1 O-glycans by GalNAc-transferases : Implications for the Pathogenesis of IgA Nephropathy2013
Author(s)
Takahashi K, Raska M, Stewart T, Stuchlova Horynova M, Hargett A, Kasperova A, Hall S, Hiki Y, Yuzawa Y, Julian BA, Moldoveanu Z, Renfrow MB, Novak J
Organizer
12th Human Proteome Organization World Congress
Place of Presentation
Yokohama, Japan
Year and Date
20130000
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[Presentation] Molecular Characterization of IgA1 Secreted by IgA1-producing Cell Lines from Patients with IgA Nephropathy2013
Author(s)
Takahashi K, Suzuki H, Yamada K, Hall S, Moldoveanu Z, Poulsen K, Kilian M, Mestecky J, Julian BA, Renfrow MB, Novak J
Organizer
13th International IgA Nephropathy Symposium
Place of Presentation
Nanjing, China
Year and Date
20130000
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[Presentation] Novak J.Synthesis of Galactose-deficient IgA1 O-glycans by GalNAc-transferases in IgA1-producing Cells : Implications for The Pathogenesis of IgA Nephropathy2013
Author(s)
Takahashi K, Raska M, Stewart T, Stuchlova Horynova M, Hargett A, Kasperova A, Hall S, Hiki Y, Yuzawa Y, Julian BA, Moldoveanu Z, Renfrow MB
Organizer
13th International IgA Nephropathy Symposium
Place of Presentation
Nanjing, China
Year and Date
20130000
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[Presentation] Identification of anti-endothelial cell antibody in patients with IgA nephropathy2013
Author(s)
Takahashi K, Kondo A, Akiyama S, Hayashi H, Koide S, Tomita M, Hasegawa M, Yoshida S, Hiki Y, Miura K, Yuzawa Y
Organizer
13th International IgA Nephropathy Symposium
Place of Presentation
Nanjing, China
Year and Date
20130000
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[Presentation] Synthesis of Galactose-Deficient IgA1 O-Glycans by GalNAc-Transferases Expressed in IgA1-Producing Cells : Implications for the Pathogenesis of IgA Nephropathy2012
Author(s)
Takahashi K, Raska M, Stuchlova-Horynova M, Kasperova A, Hall SD, Hiki Y, Yuzawa Y, Julian BA, Moldoveanu Z, Renfrow MB, Novak J
Organizer
45th Annual Meeting of the American Society of Nephrology
Place of Presentation
San Diego, CA
Year and Date
20120000
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[Presentation] Molecular Characterization of IgA1 Secreted by IgA1-Producing Cell Lines from Patients with IgA Nephropathy2012
Author(s)
Takahashi K, Suzuki H, Yamada K, Hall S, Moldoveanu, Poulsen K, Mestecky J, Julian BA, Renfrow MB, Novak J.
Organizer
45th Annual Meeting of the American Society of Nephrology
Place of Presentation
San Diego, CA
Year and Date
20120000
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[Presentation] Molecular Characterization of IgA1 Secreted by IgA1-producing Cell Lines from Patients with IgA Nephropathy2012
Author(s)
Takahashi K, Suzuki H, Yamada K, Hall S, Moldoveanu Z, Poulsen K, Kilian M, Mestecky J, Julian BA, Renfrow MB, Novak J
Organizer
60th Annual ASMS meeting
Place of Presentation
Vancouver, BC
Year and Date
20120000
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