2013 Fiscal Year Final Research Report
The roles of Nup88-protein interaction in metastasis and invasion of cancer cells.
| Project/Area Number |
24890288
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
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| Research Institution | Sojo University |
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Principal Investigator |
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| Project Period (FY) |
2012-08-31 – 2014-03-31
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| Keywords | Nup88 / がん / 分子間相互作用 / ビメンチン |
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| Research Abstract |
It has been unclear whether Nup88 overexpression contributes to the malignant progression of cancer. In this study, we screened Nup88-binidng proteins and examined the effects of the interaction on cancer progression. Using an immunoprecipitation technique, we identified vimentin, a marker for epithelial-mesenchymal transformation, as a novel binding partner for Nup88. Vimentin expression at the protein level was unaffected by Nup88 overexpression and vice versa. Nup88 interacted with the N-terminal region of vimentin and the interaction kept vimentin monomeric. Since monomeric vimentin was reported to work as a transcriptional factor, we assume that Nup88 overexpression induces cancer malignancy via modulating the vimentin-regulated gene transcription.
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