Creation of Biomaterials Endowed with Unique Properties of DNA Soft-Interfaces

2017 Fiscal Year Final Research Report

• Project/Area Number: 25220204
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: MAEDA Mizuo 国立研究開発法人理化学研究所, 前田バイオ工学研究室, 主任研究員 (10165657)
• Co-Investigator(Kenkyū-buntansha): 宝田 徹 国立研究開発法人理化学研究所, 前田バイオ工学研究室, 専任研究員 (30336010) ; 秋山 好嗣 東京理科大学, 基礎工学部教養(長万部), 講師 (40640842) ; 藤田 雅弘 国立研究開発法人理化学研究所, 前田バイオ工学研究室, 専任研究員 (50342845) ; 金山 直樹 信州大学, 総合工学系研究科(長野), 准教授(特定雇用) (80377811)
• Project Period (FY): 2013-05-31 – 2018-03-31
• Keywords: 核酸 / 生体材料 / ソフト界面 / ゲル / ナノ粒子 / コロイド / 細胞・組織 / 診断・計測

Outline of Final Research Achievements

Applications of unique colloidal behaviors of nanoparticles densely modified with DNA were successfully broadened. First, linear assemblies of fully matched double-stranded (ds) DNA-modified nanoparticles were spontaneously folded into an island-like structure to readily afford two-dimensional nanoparticle arrays on substrate surfaces. Second, nanogels covered with DNA were constructed to be used as a drug delivery carrier that released model payloads in a thermoresponsive manner. Third, force-distance curve analyses between dsDNA layers using colloid probe-AFM revealed that the outermost complementary dsDNA layers attract each other at a high NaCl concentration. Finally, gene mutation assays and discovery methods of DNA-binding drug candidates were developed using the unique DNA-surface property. All results indicate that DNA molecules are unique surface-modifiers that are capable of controlling interactions between materials surfaces.

Free Research Field

生体材料学