Exploration of Novel Markers on Cancer and Development of a Technology for the Diagnosis Utilized by Comprehensive Glycomics

2017 Fiscal Year Annual Research Report

• Project/Area Number: 25220206
• Research Institution: Hokkaido University
• Principal Investigator: 西村 紳一郎 北海道大学, 先端生命科学研究院, 教授 (00183898)
• Co-Investigator(Kenkyū-buntansha): 能祖 一裕 岡山大学, 医学部, 客員研究員 (10314668) ; 比能 洋 北海道大学, 先端生命科学研究院, 准教授 (70333333) ; 大山 力 弘前大学, 医学研究科, 教授 (80282135) ; 神山 俊哉 北海道大学, 医学研究院, 准教授 (80322816)
• Project Period (FY): 2013-05-31 – 2018-03-31
• Keywords: 糖鎖 / グライコブロッティング法 / グライコミクス / 疾患マーカー / 肝細胞癌 / 膵癌 / 腎細胞癌 / 早期診断法

Outline of Annual Research Achievements

本年度については以下の各項目において大きな進展があった。
① 新たに腎移植後の抗体関連拒絶反応を早期に予測する診断マーカー候補を同定・開発し、論文として公表した。
② 肝癌細胞株における浸潤能の変化と糖鎖変異の関係をさらに詳細に検討した。すなわち、高浸潤能を有するHLE株と低浸潤能であるHepG2間で糖鎖発現を比較したところ、11種類の糖鎖がHLEにおいて高発現していることが新たに明らかとなった。さらに細胞外マトリックスの破壊に関連するu-PAをノックダウンした場合には13種類の糖鎖発現量が低下していたがこの13種類のうち、2種の構造のみがHLEで高発現していた糖鎖と共通していた。
③ 進行食道癌の糖鎖解析により消化器癌における血清糖鎖発現の全容がほぼ明らかとなった。有望なバイオマーカー候補の一つである免疫グロブリン結合糖鎖のアガラクトシル化誘導因子として、当初癌細胞から産生されるエクソソームを想定し検討していたが、これ以外の小分子があることが判明した。
④ 一方、本研究課題は癌をはじめ多くの疾患特異的なバイオマーカーによる診断技術開発に留まらず、様々な疾患と生体内の糖鎖構造変化の関係、特に免疫バランスや恒常性維持とメタボライトとしての糖鎖構造の変調がそれらの疾患発症と進展のメカニズムとどの様な関係にあるのかについての基本的理解を深めることに大いに貢献してきた。具体的には、疾患特異的糖鎖を担持するタンパク質の動的エピトープを標的とする全く新しいタイプの抗体医薬品の研究開発に進展させた。

Research Progress Status

29年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

29年度が最終年度であるため、記入しない。

Research Products

• [Int'l Joint Research] Ludwig-Maxmilians-University Munich/Friedrich-Alexander-Universitat(ドイツ)
  • Country Name: GERMANY
  • Counterpart Institution: Ludwig-Maxmilians-University Munich/Friedrich-Alexander-Universitat
• [Int'l Joint Research] University of La Rioja(スペイン)
  • Country Name: SPAIN
  • Counterpart Institution: University of La Rioja
• [Int'l Joint Research] Addis Ababa University(エチオピア)
  • Country Name: ETHIOPIA
  • Counterpart Institution: Addis Ababa University
• [Journal Article] CLEC10A is a specific marker for human CD1c+ DCs and enhances their Toll-like receptor 7/8 induced cytokine secretion (2018)
  • Author(s): Heger L., Balk S., Luhr J., Heidkamp G.F., Lehmann C.H., Purbojo A., Hartmann A., Garcia-Martin F., Nishimura S-I, Cesnjevar R., Nimmerjahn F., Dudziak D.
  • Journal Title: Front. Immunol. Volume: 掲載確定・印刷中 Pages: 掲載確定・印刷中
  • DOI: 10.3389/fimmu.2018.00744
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Liquid biopsy of bile for the molecular diagnosis of gallbladder cancer. (2018)
  • Author(s): Kinugasa H, Nouso K, Ako S, Dohi C, Matsushita H, Matsumoto K, Kato H, Okada H.
  • Journal Title: Cancer Biol Ther. Volume: 掲載確定・印刷中 Pages: 掲載確定・印刷中
  • DOI: 10.1080/15384047.2018.1456604
  • Peer Reviewed
• [Journal Article] Transcatheter Arterial Chemoembolization to Reduce Size of Hepatocellular Carcinoma before Radiofrequency Ablation. (2018)
  • Author(s): Ako S, Nakamura S, Nouso K, Dohi C, Wada N, Morimoto Y, Takeuchi Y, Yasunaka T, Kuwaki K, Onishi H, Ikeda F, Shiraha H, Takaki A, Okada H.
  • Journal Title: Acta Med Okayama. Volume: 72 Pages: 47-52
  • DOI: 10.18926/AMO/55662
  • Peer Reviewed / Open Access
• [Journal Article] A better method for assessment of hepatic function in hepatocellular carcinoma patients treated with radiofrequency ablation: Usefulness of albumin-bilirubin grade. (2018)
  • Author(s): Hiraoka A, Kumada T, Hirooka M, Tsuji K, Itobayashi E, Kariyama K, Ishikawa T, Tajiri K, Ochi H, Tada T, Toyoda H, Nouso K, Joko K, Kawasaki H, Hiasa Y, Michitaka K
  • Journal Title: Hepatol Res. Volume: 48 Pages: E61-E67
  • DOI: 10.1111/hepr.12927
  • Peer Reviewed
• [Journal Article] Platinum-based Neoadjuvant Chemotherapy Improves Oncological Outcomes in Patients with Locally Advanced Upper Tract Urothelial Carcinoma. (2018)
  • Author(s): Hosogoe S, Hatakeyama S, Kusaka A, Hamano I, Iwamura H, Fujita N, Yamamoto H, Tobisawa Y, Yoneyama T, Yoneyama T, Hashimoto Y, Koie T, Ohyama C
  • Journal Title: Eur Urol Focus. Volume: 掲載確定・印刷中 Pages: 掲載確定・印刷中
  • DOI: 10.1016/j.euf.2017.03.013
  • Peer Reviewed
• [Journal Article] Oncologic outcomes for open and laparoscopic radical nephroureterectomy in patients with upper tract urothelial carcinoma. (2018)
  • Author(s): Kido K, Hatakeyama S, Fujita N, Yamamoto H, Tobisawa Y, Yoneyama T, Yoneyama T, Hashimoto Y, Koie T, Iwabuchi I, Ogasawara M, Kawaguchi T, Ohyama C
  • Journal Title: Int J Clin Oncol. Volume: 掲載確定・印刷中 Pages: 掲載確定・印刷中
  • DOI: 10.1007/s10147-018-1248-9
  • Peer Reviewed
• [Journal Article] Impact of bacillus Calmette-Guerin therapy of upper urinary tract carcinoma in situ: comparison of oncological outcomes with radical nephroureterectomy. (2018)
  • Author(s): Horiguchi H, Yoneyama T, Hatakeyama S, Tokui N, Sato T, Fujita N, Yamamoto H, Tobisawa Y, Yoneyama T, Hashimoto Y, Koie T, Yoshikawa K, Narita S, Kawaguchi T, Ohyama C
  • Journal Title: Med Oncol. Volume: 35 Pages: 41
  • DOI: 10.1007/s12032-018-1102-y
  • Peer Reviewed
• [Journal Article] Detecting asymptomatic recurrence after radical nephroureterectomy contributes to better prognosis in patients with upper urinary tract urothelial carcinoma. (2018)
  • Author(s): Horiguchi H, Hatakeyama S, Anan G, Kubota Y, Kodama H, Momota M, Kido K, Yamamoto H, Tobisawa Y, Yoneyama T, Yoneyama T, Hashimoto Y, Koie T, Ito H, Yoshikawa K, Kawaguchi T, Sato M, Ohyama C
  • Journal Title: Oncotarget. Volume: 9 Pages: 8746-8755
  • DOI: 10.18632/oncotarget.23982
  • Peer Reviewed
• [Journal Article] The Impact of Preoperative Severe Renal Insufficiency on Poor Postsurgical Oncological Prognosis in Patients with Urothelial Carcinoma. (2018)
  • Author(s): Momota M, Hatakeyama S, Tokui N, Sato T, Yamamoto H, Tobisawa Y, Yoneyama T, Yoneyama T, Hashimoto Y, Koie T, Narita S, Kawaguchi T, Ohyama C
  • Journal Title: Eur Urol Focus. Volume: 掲載確定・印刷中 Pages: 掲載確定・印刷中
  • DOI: 10.1016/j.euf.2018.03.003
  • Peer Reviewed
• [Journal Article] Aberrant N-Glycosylation Profile of Serum Immunoglobulins is a Diagnostic Biomarker of Urothelial Carcinomas (2017)
  • Author(s): Tanaka T., Yoneyama T., Noro D., Imanishi K., Kojima Y., Hatakeyama S., Tobisawa Y., Mori K., Yamamoto H., Imai A., Yoneyama T., Hashimoto Y., Koie T., Tanaka M., Nishimura S-I., Kurauchi S., Takahashi I., Ohyama C.
  • Journal Title: Int. J. Mol. Sci. Volume: 18 Pages: 2632
  • DOI: 10.3390/ijms18122632
  • Peer Reviewed
• [Journal Article] The use of fluoroproline in MUC1 antigen enables efficient detection of antibodies in patients with prostate cancer (2017)
  • Author(s): Somovilla V.J., Bermeijo I.A., Albuquerque I.S., Martinez S. N., Castro Lopez J., Garcia Martin F., Companon I., Hinou H., Nishimura S-I., Jimenez Barbero J., Asensio J.L, Avenoza G., Busto J.H., Hurtado-Guerrero. R., Peregrina J.M., Bernardes G.J.L., Corzana F.
  • Journal Title: J. Am. Chem. Soc. Volume: 129 Pages: 18255-18261
  • DOI: 10.1021/jacs.7b09447
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Glycopeptides as Targets for Dendritic Cells: Exploring MUC1 Glycopeptides Binding Profile toward Macrophage Galactose-Type Lectin (MGL) Orthologs (2017)
  • Author(s): Artigas G., Monteiro J.T., Hinou H., Nishimura S-I, Lepenies B., and Garcia Martin F.
  • Journal Title: J.Med.Chem. Volume: 60 Pages: 9012-9021
  • DOI: 10.1021/acs.jmedchem.7b01242
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Generation of Novel Anti-MUC1 Monoclonal Antibodies with Designed Carbohydrate Specificities Using MUC1 Glycopeptide Library (2017)
  • Author(s): Naito S., Takahashi T., Onoda J., Uemura S., Ohyabu N., Takemoto H., Yamane S., Fujii I., Nishimura S-I., Numata Y.
  • Journal Title: ACS Omega Volume: 2 Pages: 7493-7505
  • DOI: 10.1021/acsomega.7b00708
  • Peer Reviewed
• [Journal Article] Serum Aberrant N-Glycan Profile as a Marker Associated with Early Antibody-Mediated Rejection in Patients Receiving a Living Donor Kidney Transplant (2017)
  • Author(s): Noro D., Yoneyama T., Hatakeyama S., Tobisawa Y., Mori K., Hashimoto Y., Koie T., Tanaka M., Nishimura S-I., Sasaki H., Saito M., Harada H., Chikaraishi T., Ishida H., Tanabe K., Satoh S., Ohyama C.
  • Journal Title: Int. J. Mol. Sci. Volume: 18 Pages: 1731
  • DOI: 10.3390/ijms18081731
  • Peer Reviewed
• [Journal Article] An efficient glycoblotting-based analysis of oxidized lipids in liposomes and a lipoprotein (2017)
  • Author(s): Furukawa T., Hinou H., Takeda S., Chiba H., Nishimura S-I., Shu-Ping Hui
  • Journal Title: ChemBioChem Volume: 18 Pages: 1903-1909
  • DOI: 10.1002/cbic.201700384
  • Peer Reviewed
• [Journal Article] Chemical synthesis demonstrates dynamic O-glycosylation regulates folding and functional conformation of a pivotal EGF12 domain of human NOTCH1 receptor (2017)
  • Author(s): Hayakawa S., Yokoi Y., Hinou H., Nishimura S-I.
  • Journal Title: Biochemistry Volume: 56 Pages: 4379-4391
  • DOI: 10.1021/acs.biochem.7b00372
  • Peer Reviewed
• [Journal Article] Albumin-Bilirubin (ALBI) Grade as Part of the Evidence-Based Clinical Practice Guideline for HCC of the Japan Society of Hepatology: A Comparison with the Liver Damage and Child-Pugh Classifications. (2017)
  • Author(s): Hiraoka A, Kumada T, Kudo M, Hirooka M, Tsuji K, Itobayashi E, Kariyama K, Ishikawa T, Tajiri K, Ochi H, Tada T, Toyoda H, Nouso K, Joko K, Kawasaki H, Hiasa Y, Michitaka K
  • Journal Title: Liver Cancer. Volume: 6 Pages: 204-215
  • DOI: 10.1159/000452846
  • Peer Reviewed / Open Access
• [Journal Article] Role of hepatic resection in patients with intermediate-stage hepatocellular carcinoma: A multicenter study from Japan. (2017)
  • Author(s): Tada T, Kumada T, Toyoda H, Tsuji K, Hiraoka A, Itobayashi E, Nouso K, Kariyama K, Ishikawa T, Hirooka M, Hiasa Y.
  • Journal Title: Cancer Sci. Volume: 108 Pages: 1414-1420
  • DOI: 10.1111/cas.13257
  • Peer Reviewed / Open Access
• [Journal Article] Promising therapeutic efficacy of a novel reduced expression in immortalized cells/dickkopf-3 expressing adenoviral vector for hepatocellular carcinoma. (2017)
  • Author(s): Sawahara H, Shiraha H, Uchida D, Kato H, Kato R, Oyama A, Nagahara T, Iwamuro M, Horiguchi S, Tsutsumi K, Mandai M, Mimura T, Wada N, Takeuchi Y, Kuwaki K, Onishi H, Nakamura S, Watanabe M, Sakaguchi M, Takaki A, Nouso K, Yagi T, Nasu Y, Kumon H, Okada H.
  • Journal Title: J Gastroenterol Hepatol. Volume: 32 Pages: 1769-1777
  • DOI: 10.1111/jgh.13757
  • Peer Reviewed
• [Journal Article] Impact of lymph node dissection on clinical outcomes during nephroureterectomy in patients with clinically node-negative upper urinary tract urothelial cancer: subanalysis of a multi-institutional nationwide case series of the Japanese Urological Association. (2017)
  • Author(s): Inokuchi J, Eto M, Hara T, Fujimoto H, Nishiyama H, Miyazaki J, Kikuchi E, Hinotsu S, Koie T, Ohyama C
  • Journal Title: Jpn J Clin Oncol. Volume: 47 Pages: 652-659
  • DOI: 10.1093/jjco/hyx051
  • Peer Reviewed / Open Access
• [Journal Article] Post-chemotherapy PD-L1 expression correlates with clinical outcomes in Japanese bladder cancer patients treated with total cystectomy. (2017)
  • Author(s): Noro D, Hatakeyama S, Yoneyama T, Hashimoto Y, Koie T, Kawaguchi T, Ohyama C
  • Journal Title: Med Oncol. Volume: 34 Pages: 117
  • DOI: 10.1007/s12032-017-0977-3
  • Peer Reviewed
• [Journal Article] Role of lymph node dissection during radical nephroureterectomy for upper urinary tract urothelial cancer: multi-institutional large retrospective study JCOG1110A. (2017)
  • Author(s): Inokuchi J, Kuroiwa K, Kakehi Y, Sugimoto M, Tanigawa T, Fujimoto H, Gotoh M, Masumori N, Ogawa O, Eto M, Ohyama C, Yamaguchi A, Matsuyama H, Ichikawa T, Asano T, Mizusawa J, Eba J, Naito S
  • Journal Title: World J Urol. Volume: 35 Pages: 1737-1744
  • DOI: 10.1007/s00345-017-2049-x
  • Peer Reviewed
• [Journal Article] A mammalian homolog of the zebrafish transmembrane protein 2 (TMEM2) is the long-sought-after cell-surface hyaluronidase. (2017)
  • Author(s): Yamamoto H, Tobisawa Y, Inubushi T, Irie F, Ohyama C, Yamaguchi Y
  • Journal Title: J Biol Chem. Volume: 292 Pages: 7304-7313
  • DOI: 10.1074/jbc.M116.770149
  • Peer Reviewed
• [Journal Article] Detecting asymptomatic recurrence after radical cystectomy contributes to better prognosis in patients with muscle-invasive bladder cancer. (2017)
  • Author(s): Kusaka A, Hatakeyama S, Hosogoe S, Hamano I, Iwamura H, Fujita N, Fukushi K, Narita T, Yamamoto H, Tobisawa Y, Yoneyama T, Yoneyama T, Hashimoto Y, Koie T, Ito H, Yoshikawa K, Kawaguchi T, Ohyama C.
  • Journal Title: Med. Oncol. Volume: 34 Pages: 1～10
  • DOI: 10.1007/s12032-017-0955-9
  • Peer Reviewed
• [Journal Article] Risk-stratified surveillance and cost effectiveness of follow-up after radical cystectomy in patients with muscle-invasive bladder cancer. (2017)
  • Author(s): Kusaka A, Hatakeyama S, Hosogoe S, Hamano I, Iwamura H, Fujita N, Fukushi K, Narita T, Hagiwara K, Yamamoto H, Tobisawa Y, Yoneyama T, Yoneyama T, Hashimoto Y, Koie T, Ito H, Yoshikawa K, Kawaguchi T, Ohyama C
  • Journal Title: Oncotarget Volume: 8 Pages: 65492-65505
  • DOI: 10.18632/oncotarget.19043
  • Peer Reviewed
• [Journal Article] Preoperative chronic kidney disease predicts poor oncological outcomes after radical cystectomy in patients with muscle-invasive bladder cancer. (2017)
  • Author(s): Hamano I, Hatakeyama S, Iwamurau H, Fujita N, Fukushi K, Narita T, Hagiwara K, Kusaka A, Hosogoe S, Yamamoto H, Tobisawa Y, Yoneyama T, Yoneyama T, Hashimoto Y, Koie T, Ito H, Yoshikawa K, Kawaguchi T, Ohyama C
  • Journal Title: Oncotarget Volume: 8 Pages: 61404-61414
  • DOI: 10.18632/oncotarget.18248
  • Peer Reviewed
• [Journal Article] The role of LIM and SH3 protein-1 in bladder cancer metastasis. (2017)
  • Author(s): Sato M, Yoneyama MS, Hatakeyama S, Funyu T, Suzuki T, Ohyama C, Tsuboi S
  • Journal Title: Oncol Lett. Volume: 14 Pages: 4829-4834
  • DOI: 10.3892/ol.2017.6802
  • Peer Reviewed
• [Journal Article] Preoperative chronic kidney disease predicts poor oncological outcomes after radical nephroureterectomy in patients with upper urinary tract urothelial carcinoma. (2017)
  • Author(s): Kodama H, Hatakeyama S, Fujita N, Iwamura H, Anan G, Fukushi K, Narita T, Tanaka T, Kubota Y, Horiguchi H, Momota M, Kido K, Matsumoto T, Soma O, Hamano I, Yamamoto H, Tobisawa Y, Yoneyama T, Yoneyama T, Hashimoto Y, Koie T, Ito H, Yoshikawa K, Sasaki A, Kawaguchi T, Sato M, Ohyama C
  • Journal Title: Oncotarget. Volume: 8 Pages: 83183-83194
  • DOI: 10.18632/oncotarget.20554
  • Peer Reviewed
• [Journal Article] Trends in neoadjuvant chemotherapy use and oncological outcomes for muscle-invasive bladder cancer in Japan: a multicenter study. (2017)
  • Author(s): Anan G, Hatakeyama S, Fujita N, Iwamura H, Tanaka T, Yamamoto H, Tobisawa Y, Yoneyama T, Yoneyama T, Hashimoto Y, Koie T, Ito H, Yoshikawa K, Kawaguchi T, Sato M, Ohyama C
  • Journal Title: Oncotarget. Volume: 8 Pages: 86130-86142
  • DOI: 10.18632/oncotarget.20991
  • Peer Reviewed
• [Journal Article] Significance of preoperative butyrylcholinesterase level as an independent predictor of survival in patients with upper urinary tract urothelial carcinoma treated with nephroureterectomy. (2017)
  • Author(s): Noro D, Koie T, Hashimoto Y, Tanaka T, Ohyama C, Tobisawa Y, Yoneyama T, Imai A, Hatakeyama S, Yamamoto H, Kitayama M, Hirota K
  • Journal Title: Jpn J Clin Oncol Volume: 48 Pages: 184-189
  • DOI: 10.1093/jjco/hyx168
  • Peer Reviewed
• [Journal Article] Oncological outcomes of neoadjuvant chemotherapy in patients with locally advanced upper tract urothelial carcinoma: a multicenter study. (2017)
  • Author(s): Kubota Y, Hatakeyama S, Tanaka T, Fujita N, Iwamura H, Mikami J, Yamamoto H, Tobisawa Y, Yoneyama T, Yoneyama T, Hashimoto Y, Koie T, Ito H, Yoshikawa K, Sasaki A, Kawaguchi T, Ohyama C
  • Journal Title: Oncotarget. Volume: 8 Pages: 101500-101508
  • DOI: 10.18632/oncotarget.21551
  • Peer Reviewed
• [Presentation] 独創的なシステム糖鎖工学プラットフォームからの創薬イノベーション (2018)
  • Author(s): 西村 紳一郎
  • Organizer: 医薬基盤・健康・栄養研究所セミナー
  • Invited
• [Presentation] 網羅的血清糖鎖分析による生体腎移植後拒絶反応予測マーカーの開発 (2018)
  • Author(s): 畠山真吾，米山 徹，齋藤 満，佐藤 滋，原田 浩，石田英樹，田邉一成，佐々木秀郎，力石辰也，大山 力
  • Organizer: 第27回泌尿器科分子・細胞研究会
• [Presentation] 癌性糖鎖変異S2，3PSA検査の測定安定性と既存PSA検査の前立腺癌診断 (2018)
  • Author(s): 米山 徹，石川友一，飛澤悠葵，畠山真吾，成田伸太郎，三塚浩二，羽渕友則，荒井陽一，伊達睦廣，大山 力
  • Organizer: 第27回泌尿器科分子・細胞研究会
• [Presentation] PSA糖鎖修飾異性体（PSA-Gi）検査と既存PSA検査との前立腺癌診断能比較 (2018)
  • Author(s): 米山 徹，飛澤悠葵，金子智典，彼谷高敏，畠山真吾，三塚浩二，橋本安弘，荒井陽一，須田美彦，大山 力
  • Organizer: 第27回泌尿器科分子・細胞研究会
• [Presentation] PSA glycoisoform (PSA-Gi) と既存PSA検査による前立腺癌の診断能の比較 (2017)
  • Author(s): 米山 徹，飛澤悠葵，金子智典，彼谷高敏，畠山真吾，今井 篤，米山高弘，橋本安弘，古家琢也，須田美彦，大山 力
  • Organizer: 第33回前立腺シンポジウム
• [Presentation] MECA79 positive high endotherial venule density (HEV) correalates with clinical outcomes in Upper Tract Urothelial Carcinoma patients treated with Radical Nephroureterectomy (2017)
  • Author(s): Hashimoto Y, Yoneyama T, Imai A, Hatakeyama S, Yoneyama T, Koie T, Ohyama C
  • Organizer: ESMO ASIA 2017
  • Int'l Joint Research
• [Presentation] 癌性糖鎖変異PSAと既存PSA検査による前立腺癌の診断精度の比較 (2017)
  • Author(s): 米山 徹，飛澤悠葵，金子智典，彼谷高敏，畠山真吾，今井 篤，米山高弘，橋本安弘，古家琢也，須田美彦，大山 力
  • Organizer: 東北糖鎖研究会・東京糖鎖研究会合同シンポジウム（第11回東北糖鎖研究会・Glyco TOKYO 2017）
• [Presentation] O-グリカン修飾変化を利用した新規CTL腫瘍免疫逃避機構 (2017)
  • Author(s): 米山美穂子，飛澤悠葵，畠山真吾，佐藤美紗季，刀禰亀代志，多田羅洋太，柿崎育子，舟生富寿，福田 穰，星 宣次，大山 力，坪井 滋
  • Organizer: 東北糖鎖研究会・東京糖鎖研究会合同シンポジウム（第11回東北糖鎖研究会・Glyco TOKYO 2017）
• [Presentation] Transmembrane protein 2 (TMEM2) は細胞表面においてヒアルロン酸を分解する (2017)
  • Author(s): 山本勇人，飛澤悠葵，犬伏俊博，入江史俊，畠山真吾，米山 徹，米山高弘，橋本安弘，古家琢也，大山 力，山口 祐
  • Organizer: 東北糖鎖研究会・東京糖鎖研究会合同シンポジウム（第11回東北糖鎖研究会・Glyco TOKYO 2017）
• [Presentation] 動的エピトープ理論：タンパク質のダイナミックな翻訳後修飾が創出する疾患特異的抗原構造 (2017)
  • Author(s): 西村 紳一郎
  • Organizer: 第57回日本臨床化学会年次学術集会
• [Presentation] 癌性糖鎖変異PSA-Giと既存PSA検査による前立腺癌の診断能の比較 (2017)
  • Author(s): 米山 徹、飛澤悠葵、金子智典、仮谷高敏、畠山真吾、今井 篤、米山高弘、橋本安弘、古家琢也、須田美彦、大山 力
  • Organizer: 第82回日本泌尿器科学会東部総会
• [Presentation] 腎移植患者における血清N型糖鎖の変化についての検討 (2017)
  • Author(s): 畠山真吾，米山 徹，野呂大輔，佐藤 滋，力石辰也，石田英樹，田邉一成，原田 浩，大山 力
  • Organizer: 第53回日本移植学会総会
• [Presentation] Nanomedicine targeting intracellular glycoenzymes (2017)
  • Author(s): 西村 紳一郎
  • Organizer: Gordon Research Conference (GRC) 2017
  • Int'l Joint Research / Invited
• [Presentation] Clinical implications of serum N-glycanprofiling as a diagnostic and prognostic biomarker in germ-cell tumors． (2017)
  • Author(s): Narita T, Hatakeyama S, Yoneyama T, Narita S, Yamashita S, Mitsuzuka K, Sakurai T, Kawamura S, Tochigi T, Takahashi I, Nakaji S, Tobisawa Y, Yamamoto H, Koie T,Tsuchiya N, Habuchi T, Arai Y, Ohyama C
  • Organizer: 第112回米国泌尿器科学会議(AUA 2017)
  • Int'l Joint Research
• [Presentation] Serum N-glycomics predicts in patients who developed castration resistant prostate cancer． (2017)
  • Author(s): Yoneyama T, Tobisawa Y, Hatakeyama S, Mori K, Hashimoto Y, Koie T, Ohyama C
  • Organizer: 第112回米国泌尿器科学会議(AUA 2017)
  • Int'l Joint Research
• [Presentation] Significance of serum N-glycan profiling as a diagnostic biomarker in urothelial carcinoma． (2017)
  • Author(s): Oikawa M, Hatakeyama S, Yoneyma T, Tobisawa Y, Narita T, Yamamoto H, Hashimoto Y, Koie T, Narita S, Tsuchiya N, Habuchi T, Takahashi I, Nakaji S, Ohyama C
  • Organizer: 第112回米国泌尿器科学会議(AUA 2017)
  • Int'l Joint Research
• [Presentation] Serum sialyl hybrid typed N-glycan levels predicts early ABMR in living donor kidney transplant patients． (2017)
  • Author(s): Noro D, Yoneyama T, Hatakeyama S, Tobisawa Y, Mori K, Hashimoto Y, Koie T, Tanaka M, Nishimura S, Sasaki H, Saito M, Harada H, Chikaraishi T, Ishida H, Tanabe K, Satoh S, Ohyama C
  • Organizer: 第112回米国泌尿器科学会議(AUA 2017)
  • Int'l Joint Research
• [Presentation] Serum sialyl hybrid typed N-glycan levels predicts early ABMR in living donor kidney transplant patients． (2017)
  • Author(s): Tanaka T, Tobisawa Y, Hatakeyama S, Mori K, Hashimoto Y, Koie T, Ohyama C, Yoneyama T.
  • Organizer: 第112回米国泌尿器科学会議(AUA 2017)
  • Int'l Joint Research
• [Presentation] 網羅的糖鎖解析による肝細胞癌の浸潤能と糖鎖異常の検討 (2017)
  • Author(s): 高橋 秀徳, 神山 俊哉, 柿坂 達彦, 相山 健, 島田 慎吾, 若山 顕治, 永生 高弘, 折茂 達也, 蒲池 浩文, 横尾 英樹, 西村 紳一郎, 武冨 紹信
  • Organizer: 第117回日本外科学会定期学術集会
• [Presentation] マイクロキャピラリー電気泳動免疫蛍光検出装置を用いた新規の前立腺癌関連糖鎖変異マーカーS2,3PSA 測定に基づく診断手法 (2017)
  • Author(s): 石川友一、米山 徹、飛澤悠葵、畠山真吾、黒澤竜雄、橋本安弘、古家琢也、中村賢治、大山 力
  • Organizer: 第105回日本泌尿器科学会総会
• [Presentation] 血清N-結合型糖鎖の網羅的質量解析による精巣腫瘍の診断・予後マーカーの検索 (2017)
  • Author(s): 成田拓磨、畠山真吾、米山 徹、成田伸太郎、山下慎一、櫻井俊彦、飛澤悠葵、古家琢也、土谷順彦、羽渕友則、荒井陽一、大山 力
  • Organizer: 第105回日本泌尿器科学会総会
• [Presentation] 尿路上皮癌症例における新規バイオマーカーとしての血清N-glycan の網羅的質量解析 (2017)
  • Author(s): 及川真亮、畠山真吾、成田拓磨、今井 篤、米山高弘、米山 徹、橋本安弘、古家琢也、大山 力
  • Organizer: 第105回日本泌尿器科学会総会
• [Presentation] New glycotechnology platform interfacing chemistry and biology (2017)
  • Author(s): 西村 紳一郎
  • Organizer: 253rd ACS National Meeting
  • Int'l Joint Research / Invited