2017 Fiscal Year Final Research Report
Molecular pathways leading to epigenome formation in mammalian germ cells
| Project/Area Number |
25221003
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| Research Category |
Grant-in-Aid for Scientific Research (S)
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| Allocation Type | Single-year Grants |
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| Research Field |
Genome biology
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| Research Institution | Keio University |
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Principal Investigator |
SIOMI HARUHIKO 慶應義塾大学, 医学部(信濃町), 教授 (60202107)
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| Research Collaborator |
HASUWA Hidetoshi
IWASAKI Yuka
YAMANAKA Soichiro
MURANO Kensaku
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| Project Period (FY) |
2013-05-31 – 2018-03-31
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| Keywords | 生殖細胞 / エピジェネティクス / クロマチン / トランスポゾン / Piwi / piRNA |
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| Outline of Final Research Achievements |
The PIWI-piRNA pathway is a conserved cellular pathway that represses transposable elements (TEs) and plays an important role in germline development. We have recently generated PIWI KO hamster (Syrian hamster; Mesocricetus auratus) in which three of its four distinct PIWIs are expressed in the ovary and found that PIWI KO female are sterile.We have also found novel chromatin domains termed DADs (Differentially Accessible Domains) which span more than mega bases, get transiently accessible in embryonic male germ cells right after the reprogramming event, and turn back to closed state during gonocyte. Interestingly, TEs located in DAD become activated during mid-gonocyte stages.
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| Free Research Field |
複合領域
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