Development and pharmacokinetic application of an intestine and liver in vitro model derived from ES/iPS cells

2016 Fiscal Year Final Research Report

• Project/Area Number: 25242040
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: Tagawa Yoh-ichi 東京工業大学, 生命理工学院, 准教授 (70262079)
• Co-Investigator(Renkei-kenkyūsha): FUJIYAMA Yoichi 株式会社島津製作所, デバイス部, グループ長 (80396093)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 腸 / 肝 / 臓器チップ / 代謝試験 / 毒性試験 / 動物実験代替法 / マイクロ流体デバイス

Outline of Final Research Achievements

In mammals, digested substances taken from mouth were incorporated and metabolized at the intestine, and then were delivered to liver through hepatic portal vein. Liver plays crucial roles such as energy source accumulation and supply to whole body, detoxification and excretion by conjugation. Because, for drug development, it is necessary to investigate kinetics, metabolism, and toxicity of drugs in intestine and liver, many animal experiments and clinical studies have been carried out. So, we succeeded to establish ES/iPS-derived in vitro drug metabolism assay system closed to real intestine and liver, and these ES/iPS-derived intestine and liver tissues were cultured on our original fluidic devices. These results must be expected to be animal experiment alternatives.

Free Research Field

合成生物学