Cryopreservation of the stem cell and tissue for regenerative medicine with antifreeze polyamino-acid

2015 Fiscal Year Final Research Report

• Project/Area Number: 25242050
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Medical systems
• Research Institution: Kyoto Institute of Technology
• Principal Investigator: Gen Syokyu 京都工芸繊維大学, 繊維科学センター, 教授 (90283655)
• Co-Investigator(Kenkyū-buntansha): MATSUMURA Kazuaki 北陸先端科学技術大学院大学, マテリアルサイエンス研究科, 准教授 (00432328) ; AKATANI Akiko (AKATANI Akiko) 兵庫医科大学, 医学部, 助教授 (50212402) ; YOSHIDA Yoshiko 富山大学, 大学院医学薬学研究部, 准教授 (00171421) ; OKABE Motonori 富山大学, 大学院医学薬学研究部, 助教 (60283066)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 凍結保存 / ガラス化保存 / 緩慢凍結 / 組織 / 細胞 / 受精卵

Outline of Final Research Achievements

Currently, certain realization is expected about regenerative medicine. However, research of them is not concentrated on so much by the technology of preserving tissue currently, in order to focus on creating reproduction tissue material. Then, we performed the research and development of the novel cryopreservation technology of various tissues. In development of new cryopreservation, It found out the possibility of the "new vitrification freeze method of preservation." In addition, we obtained the result which produces the result more than fixed in preservation of a fertilized ovum, umbilical cord blood, etc., and leads to future. Furthermore, in preservation of a cell sheet, it proved that new cryopreservation liquid has the preservation capability more than equivalent as compared with the conventional method. It was shown that the new cryopreservation liquid which solves toxic problems, such as dimethylsulfoxide used conventionally, has the possibility to clinical application.

Free Research Field

細胞・組織凍結保存