2016 Fiscal Year Final Research Report
Studies for homologous recombination in chromatin
| Project/Area Number |
25250023
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Genome biology
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| Research Institution | Waseda University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
清水 光弘 明星大学, 理工学部, 教授 (80231364)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Keywords | ゲノム維持修復 / ゲノム多様性 / ゲノム進化・再編 / 遺伝情報複製・再編 / ゲノム機能 / 活性発現の分子機構 / 染色体構築・機能・分配 / 生体高分子構造・機能 |
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| Outline of Final Research Achievements |
The purpose of this study is to elucidate the molecular mechanism of homologous recombination reactions in the higher-ordered chromatin. In this study, we established the homologous pairing assay in nucleosomal template in vitro, and clarified the mechanism of homologous pairing reactions in in the higher-ordered chromatin. In addition, we found that the chromatin structure containing histone variants regulates the homologous pairing activities. Furthermore, we also found the functional differences of RAD51 and DMC1, which are key enzymes in homologous recombination.
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| Free Research Field |
生化学、構造生物学、分子生物学
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