Next-generation single-molecule biophysics on ATP synthase

2015 Fiscal Year Final Research Report

• Project/Area Number: 25251016
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biophysics
• Research Institution: The University of Tokyo
• Principal Investigator: Noji Hiroyuki 東京大学, 工学(系)研究科(研究院), 教授 (00343111)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 1分子生物物理 / ナノバイオ / ATP合成酵素 / 生体エネルギー / マイクロチャンバー

Outline of Final Research Achievements

This project made two major achievements. The first is elucidation of molecular mechanism of F1-ATPase. The second is the development of novel single-molecule measurement techniques. The achievements in the first category include the elucidation of self-inactivation of F1-ATPase that is physiologically relevant. In collaboration with theorists, we revealed that angles for hydrolysis and inorganic phosphate release are different that have been thought to be the same. We also revealed the interaction of F1 with phosphate group of ATP directly leads torque generation. The novel single-molecule techniques developed in this project are nanorod imaging with ultra high spatiotemporal resolution and arrayed lipid bilayer chamber system (ALBiCs). Both of novel techniques were proved to be very powerful to bring new findings that have been not accessible with previous single molecule techniques.

Free Research Field

1分子生物物理