Comprehensive analysis of self-incompatibility downstream genes by using SI Arabidopsis

2015 Fiscal Year Final Research Report

• Project/Area Number: 25252001
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Science in genetics and breeding
• Research Institution: Tohoku University
• Principal Investigator: Watanabe Masao 東北大学, 生命科学研究科, 教授 (90240522)
• Co-Investigator(Kenkyū-buntansha): SUWABE KEITA 三重大学, 大学院生物資源学研究科, 准教授 (50451612) ; YANO KANTARO 明治大学, 農学部, 教授 (00446543)
• Project Period (FY): 2013-10-21 – 2016-03-31
• Keywords: 遺伝学 / 育種学 / アブラナ科植物 / 自家不和合性 / 情報伝達 / シロイヌナズナ / アブラナ / バイオインフォマティクス

Outline of Final Research Achievements

In order to determine the SRK downstream candidate gene, QTL and comprehensive expression analysis in the self-compatible line were performed. As a candidate gene, we identified a gene having sequence similarity to resistance gene to pathogen. Now, we are establishing the disruptant lines for candidate gene. We will determine new SRK downstream gene. By using self-incompatible Arabidopsis thaliana line, main factor to self-compatibility was inversion in the second exon. In addition to this point, the second self-compatibility factor was lower activity of promoter sequence against to self-incompatible Arabidopsis relatives. Furthermore, we established genome-wide gene expression profile from the next generation sequencer data. These data will contribute to identify the SRK downstream candidate genes and other pollen-stigma interaction related genes. In addition to the experiments, we also performed the outreach activities to elementary school, high school students.

Free Research Field

植物分子育種学