2016 Fiscal Year Final Research Report
Antiviral innate immunity in phytopathogenic fungi
Project/Area Number |
25252011
|
Research Category |
Grant-in-Aid for Scientific Research (A)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Plant protection science
|
Research Institution | Okayama University |
Principal Investigator |
SUZUKI Nobuhiro 岡山大学, 資源植物科学研究所, 教授 (70206514)
|
Co-Investigator(Kenkyū-buntansha) |
中屋敷 均 神戸大学, (連合)農学研究科(研究院), 教授 (50252804)
近藤 秀樹 岡山大学, その他部局等, 准教授 (40263628)
|
Project Period (FY) |
2013-04-01 – 2017-03-31
|
Keywords | 植物病原糸状菌 / RNAサイレンシング / RNAi / マイコウイルス / dsRNA / 自然免疫 / マイコイミュニティ / 抗ウイルス防御反応 |
Outline of Final Research Achievements |
The objective of this project is to explore RNA silencing (RNAi) as the antiviral innate defense against fungal viruses in plant pathogenic filamentous fungi. Consequently, a great progress has been made, leading to breakthroughs: 1) Discovery of RNAi mediated antiviral defense that requires Dicer but not AGO in Cryphonectria parasitica, 2) Detection of inhibitory effects on RNA silencing activities against RNA and DNA viruses, and transgenes in Pyricularia oryzae, 3) Development of a screening protocol for host factors required for virus perception through transcription induction of key genes of RNAi upon virus infection in C. parasitica, 4) Identification of the SAGA complex as a transcriptional regulator of fungal RNA silencing. These findings will bring about a paradigm shift in RNAi. Fungi are now established as the third position next to animals and plants in antiviral defense research. Note that most of the obtained data were published in international, high-quality journals.
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Free Research Field |
植物病理学
|