Spatio-temporal regularory mechanisms of steroid uptake and transport in insect steroid hormone producing organs.

2017 Fiscal Year Final Research Report

• Project/Area Number: 25252023
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bioorganic chemistry
• Research Institution: The University of Tokyo
• Principal Investigator: Kataoka Hiroshi 東京大学, 大学院新領域創成科学研究科, 教授 (60202008)
• Research Collaborator: OGIHARA Mari 学術振興会, RPD特別研究員
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: ステロイド / コレステロール / 前胸腺 / カイコ

Outline of Final Research Achievements

LC-MS/MS analyses showed that cholesterol and 7-dehydrocholesterol increased with developmental growth in the prothoracic glands where the insect steroid hormone, ecdysone, was produced. The results of multivariate analysis of steroids compared with other tissues showed that the prothoracic glands have different steroid profiling from other tissues. Furthermore, the localization analyses of lipophorin, a steroid transport lipoprotein, and cholesterol also supported the existence of a steroid uptake mechanism unique to the prothoracic glands. We also could identify one scavenger receptor which might be a molecule involved in steroid uptake from lipophorin in the prothoracic glands.

Free Research Field

分子認識化学