Prediction of deterioration rate of fruits and vegetables on the basis of visualized localization of enzymatic activity for respiration

2017 Fiscal Year Final Research Report

• Project/Area Number: 25252045
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Agricultural environmental engineering/Agricultural information engineering
• Research Institution: The University of Tokyo
• Principal Investigator: Makino Yoshio 東京大学, 大学院農学生命科学研究科(農学部), 准教授 (70376565)
• Co-Investigator(Kenkyū-buntansha): 野口 航 東京薬科大学, 生命科学部, 教授 (80304004)
• Co-Investigator(Renkei-kenkyūsha): OSHITA Seiichi 東京大学, 大学院農学生命科学研究科, 特任教授 (00115693)
• Research Collaborator: TSUKADA Masato 日本電気株式会社, データサイエンス研究所, 主任研究員 ; PARK Bosoon 米国農務省, Richard B. Russell Research Center, Lead scientist
• Project Period (FY): 2013-04-01 – 2018-03-31
• Keywords: 分光分析 / 農業工学 / 農林水産物 / 細胞・組織 / 酵素

Outline of Final Research Achievements

We studied the distribution of deterioration rate of cruciferous vegetable flower buds and the relationship between respiratory enzymes. By the light sensing, the nondestructive estimation accuracy of the yellowing rate distribution of broccoli flower buds was influenced by light reflectance around 750 nm, which was confirmed to be light reflection due to chlorophyll fluorescence. Nondestructive estimation of the browning rate distribution of cauliflower flower buds was possible with screening level accuracy.
The yellowing rate of the broccoli flower bud was deeply related to the increase / decrease of one type of respiratory enzyme "Alternative Oxidase (AOX)", and the yellowing was suppressed under the environmental condition where the increase in AOX amount was suppressed. From this, it became clear that respiratory enzymes influence the deterioration rate control of cruciferous vegetables.

Free Research Field

農業環境・情報工学