Regulation of developmental switiching of inhibitory circuits

2016 Fiscal Year Final Research Report

• Project/Area Number: 25253017
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General physiology
• Research Institution: National Institute for Physiological Sciences
• Principal Investigator: NABEKURA Junichi 生理学研究所, 基盤神経科学研究領域, 教授 (50237583)
• Co-Investigator(Kenkyū-buntansha): 福田 敦夫 浜松医科大学, 医学部, 教授 (50254272)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: グリシン受容体 / イメージング / スイッチング / シナプス

Outline of Final Research Achievements

We attempted to investigate the postsynaptic receptor dynamics during the formation of glycinergic inhibitory synapses by using electrophysiological and single molecular imaging approach. In spinal cord neurons cultured in the presence of strychinine, a glycine receptor antagonist, miniature inhibitory synaptic currents gradually increased in amplitude after wash-out strychinine. In addition, glycine tagged with Q dot moved much slower at the synaptic sites without strychinine compared with that with the receptor antagonist. In addition, lateral mobility of glycine receptor could be accelerated without glycine receptor. This suggests that glycine receptor at the inhibitory synapses could be limited in the mobility, resulting in the accumulation of glycine receptor at the glycinergic synapses. PKC activation upon the glycine receptor activation might regulate the receptor accumulation.

Free Research Field

一般生理学