2015 Fiscal Year Final Research Report
Long ncRNA expression abnormality in polyglutmine diseases
| Project/Area Number |
25253066
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurology
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| Research Institution | Doshisha University (2014-2015)
Juntendo University (2013) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
Miyazaki Haruko 独立行政法人理化学研究所, 視床発生研究チーム, 研究員 (80525890)
下郡 智美 独立行政法人理化学研究所, 視床発生研究チーム, チームリーダー (30391981)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | ポリグルタミン病 / 遺伝子発現 / lncRNA |
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| Outline of Final Research Achievements |
To reveal the pathological significance of the gene expression abnormality in Huntington disease(HD), we developed the method to identify the gene expression changes in specific neuronal fraction such as medium spiny neurons(MSN) in striatum. We isolated MSN using cell sorter from control and HD model mice with Venus expression in MSN, then compared their gene expressions with microarray. We identified many genes, which showed expression changes, those include previously reported and unreported ones.Those could be confirmed by RT-PCR if the expression levels are above the certain level. We also identified the expression abnormality of long non-coding RNA, however, many of them showed low expression level. Thus, new methods for confirmation is necessary.
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| Free Research Field |
病態脳科学
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