Generation of pancreatic islet-like tissue by using an innovative 3D-culture method.

2016 Fiscal Year Final Research Report

• Project/Area Number: 25253079
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General surgery
• Research Institution: Yokohama City University
• Principal Investigator: TANIGUCHI Hideki 横浜市立大学, 医学研究科, 教授 (70292555)
• Co-Investigator(Kenkyū-buntansha): 関根 圭輔 横浜市立大学, 医学部, 助教 (00323569) ; 武部 貴則 横浜市立大学, 医学部, 准教授 (20612625) ; 小池 直人 横浜市立大学, 医学(系)研究科(研究院), 客員教授 (50301081)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 膵臓 / 膵島移植 / 三次元培養 / 血管

Outline of Final Research Achievements

We are developing a scaffold-free 3D-human organ bud generation method　from reproduction of interaction with endothelial and mesenchymal cells mimicking initial process of organogenesis (Nature　499:481-484, 2013). Adapting this 3D-culture system, we have tried to develop a cell manipulation technique to artificially build a 3D pancreatic islet tissue using pancreas β cells. We have succeeded in condensation of multiple pancreatic islet and forming vascular-like structures around it by developing multiple clustering technology and promotion technology of vascularization targeting mice and human pancreatic islet. By transplanting this vascularized islet to immune-deficient mice, the same level of vasculature as pancreatic islet in vivo is maintained inside the vascularized pancreatic islet. The vascularized islets was transplanted into fulminant type 1 diabetes mellitus modeled mice resulted in significant improvement of survival rate of these mice.

Free Research Field

移植外科学