2015 Fiscal Year Final Research Report
Alternative DNA repair pathways of DNA damage induced by topoisomerase inhibitor
| Project/Area Number |
25281018
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation and chemicals
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| Research Institution | Osaka University |
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Principal Investigator |
Kuraoka Isao 大阪大学, 基礎工学研究科, 准教授 (60335396)
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| Co-Investigator(Renkei-kenkyūsha) |
Iwai Shigenori 大阪大学, 大学院基礎工学研究科, 教授 (10168544)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | トポイソメラーゼ阻害剤 / 癌 / DNA修復 |
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| Outline of Final Research Achievements |
Human topoisomerase inhibitors are effective against a broad spectrum of tumors. They induce DNA damage leading cell death by the inhibition of enzymatic topoisomerase reaction specifically.
Here, we studied the repair mechanism of topoisomerase-induced DNA damage in human cells. We found that ERCC1-XPF and RPA participates in DNA repair of the Top1-DNA damage complex and that FEN1 participates in repair of the 5'-phosphotyrosyl terminus of DNA single-strand breaks. Our data provided insights into the DNA repair mechanisms in tumor cells.
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| Free Research Field |
放射線・化学物質影響科学
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