2016 Fiscal Year Final Research Report
Androgen-induced signaling and sexual differentiation of reproductive organs
| Project/Area Number |
25281026
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation and chemicals
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| Research Institution | Gifu Pharmaceutical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
永瀬 久光 岐阜薬科大学, 薬学部, 教授 (40141395)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Keywords | アンドロゲン / 性分化 / 17β-ヒドロキシステロイド脱水素酵素 / 胎生期・発達期影響 / トランスジェニックマウス |
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| Outline of Final Research Achievements |
Androgens play a major role in male sexual development. Although androgen-induced masculinization has been well studied, genuine role of androgens in reproductive development remains unclear. To study the direct effect of androgens in reproductive development, we generated a transgenic mouse that carries a transgene expressing EGFP-tagged 17β-hydroxysteroid dehydrogenase type 3 (17β3E), which converts androstenedione into testosterone, based on the Cre/loxP recombination (17β3ETG mouse). When 17β3ETG mice were mated with Cre-expressing TG mice, testosterone and dihydrotestosterone levels in the 17β3E/Cre double TG fetuses (DTG) were significantly higher than those of the 17β3E single TG littermates in both male and female. Consistent with these results, female DTG has formed male reproductive tracts in the prenatal and postnatal period. These results suggest that our model mice are potential tool for investigating genuine role of androgens in reproductive development.
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| Free Research Field |
分子毒性学
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