2015 Fiscal Year Final Research Report
Establishment of ESR imaging methods for evaluation of skin diseases
| Project/Area Number |
25282124
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Hirosaki University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SAWAMURA DAISUKE 弘前大学, 大学院医学研究科, 教授 (60196334)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 電子スピン共鳴 / フリーラジカル / 生体計測 / イメージング / 皮膚科学 |
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| Outline of Final Research Achievements |
We investigated identification and locations of abnormality of the stratum corneum (SC) in patients with psoriasis vulgaris (PV) by using 9 GHz electron spin resonance (ESR) imaging. The pieces of the SC and PV-SC were incubated in an aqueous solution of a spin probe for about 60 minutes at 37 oC. The probe solution was dropped on the sample sheet. The SC sheet repels the aqueous solution but the probe goes into the lipid phase during the incubation. After rinsing with distilled water to remove excess spin probe, the sample was mounted on an ESR cell and was measured by the ESR spectrometer. All measurements were performed at room temperature.
The strong red signal is due to probe penetration into the PV skin. No red lesion region was observed in the control. The two-dimensionalimages showed various sizes and number distribution concerning the disordered states in the SC structure. Thus, 9 GHz ESR imaging can be useful for identifying the location of abnormality of the SC states.
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| Free Research Field |
生物物理化学
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