2015 Fiscal Year Final Research Report
Mechanoresponses of endothelial cell membranes to fluid shear stress
Project/Area Number |
25282128
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Biomedical engineering/Biomaterial science and engineering
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Research Institution | The University of Tokyo |
Principal Investigator |
Yamamoto Kimiko 東京大学, 医学(系)研究科(研究院), 講師 (00323618)
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Co-Investigator(Kenkyū-buntansha) |
ANDO Joji 獨協医科大学, 医学部, 特任教授 (20159528)
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Keywords | 流れずり応力 / 血管内皮細胞 / 細胞膜 / コレステロール / 伸展張力 / メカノトランスダクション / メカノセンシング / 細胞増殖因子受容体 |
Outline of Final Research Achievements |
Vascular endothelial cells (ECs) respond to the hemodynamic shear stress and stretch by altering their morphology, functions, and gene expression. Here we conclude that plasma membranes differentiate between stretch and shear stress by undergoing transitions in their lipid phases. Shear stress decreased the lipid order of EC plasma membranes; stretching increased the membrane lipid order. Similar changes occurred in the artificial lipid bilayer membranes, indicating that these are physical phenomena. Blocking these changes in the membrane lipid order by depleting membrane cholesterol with MβCD or by adding cholesterol resulted in marked inhibitions of the stretch or shear stress induced phosphorylation of PDGF receptors or VEGF receptors, respectively. These results indicate that EC membranes differently respond to hemodynamic forces and that the changes in membrane physical properties are involved in the mechanotransduction that activates membrane receptors by each force.
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Free Research Field |
バイオメカニクス
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