2016 Fiscal Year Final Research Report
Single cell biomechanics and the investigation of mechanical field in cell nucleus
Project/Area Number |
25282133
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Biomedical engineering/Biomaterial science and engineering
|
Research Institution | Aino University (2016) Osaka University (2013-2015) |
Principal Investigator |
|
Project Period (FY) |
2013-04-01 – 2017-03-31
|
Keywords | 細胞 / 細胞核 / ストレスファイバー / 力学的特性 / 力学場 |
Outline of Final Research Achievements |
In the micropipette aspiration test of a single cell, elastic modulus of isolated nucleus was about 4 times higher than that of a whole cell. While, in the compressive test of a single cell, elastic modulus of isolated nucleus was about 2 times higher than that of a whole cell. From the averaged elastic modulus of the nuclei of fibroblasts and change in averaged height of nuclei in adherent fibroblasts before and after F-actin/stress fiber disruption, compressive stress imposed on nuclei by stress fibers in adherent fibroblasts was estimated to be about 0.8 kPa.
|
Free Research Field |
細胞バイオメカニクス
|