Development of Tissue Engineering for Vascularization based on regulation of interstitial flow

2015 Fiscal Year Final Research Report

• Project/Area Number: 25282135
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Keio University
• Principal Investigator: SUDO RYO 慶應義塾大学, 理工学部, 准教授 (20407141)
• Co-Investigator(Kenkyū-buntansha): TANISHITA KAZUO 早稲田大学, ナノ・ライフ創新研究機構, 上級研究員(研究院教授) (10101776) ; MITAKA TOSHIHIRO 札幌医科大学, 医学部, 教授 (50231618) ; USHIYAMA AKIRA 国立保健医療科学院, 生活環境研究部, 上席主任研究官 (60291118)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: マイクロ流体デバイス / 肝臓 / 血管

Outline of Final Research Achievements

It is necessary to reconstruct three-dimensional (3D) complex organs in current tissue engineering. In particular, construction 3D complex tissues including microvascular networks is a big challenge. In this study, we investigated a technology to vascularize 3D hepatocyte tissues. Specifically, we could construct 3D hepatocyte tissues with microvessels by controlling the timing of vascular formation and hepatocyte tissue formation in 3-channel microfluidic devices. Furthermore, we demonstrated hepatocyte-conditioned medium and collagen gel microspheres could promote vascularization of hepatocyte tissues.

Free Research Field

組織工学、生体工学