Construction of cell culture environment based on regulation of orientation and denaturation of adsorbed proteins by molecular mobility

2015 Fiscal Year Final Research Report

• Project/Area Number: 25282142
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Yui Nobuhiko 東京医科歯科大学, 生体材料工学研究所, 教授 (70182665)
• Co-Investigator(Kenkyū-buntansha): TAMURA ATSUSHI 東京医科歯科大学, 生体材料工学研究所, 助教 (80631150) ; ARISAKA YOSHINORI 東京医科歯科大学, 生体材料工学研究所, 助教 (70590115) ; SEO JI-HUN 東京医科歯科大学, 生体材料工学研究所, 助教 (20611544)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: ポリロタキサン / 分子運動性 / 細胞機能調節

Outline of Final Research Achievements

For constructing biocompatible environments using molecularly mobile surfaces based on polyrotaxane (PRX), we fabricated PRX-coated surfaces and evaluated the effect of the molecular mobility on protein adsorption and the differentiation of stem cells on PRX surfaces. By regulating Rho-family GTPases depending on molecular mobility of PRX surfaces, mesenchymal stem cells (MSCs) on PRX surface with lowly molecular mobility were introduced the differentiation into an osteoblast linage, and those on PRX surfaces with highly mobile surfaces were introduced the differentiation into an adipocyte lineage. In addition, induced pluripotent stem (iPS) cells on PRX surface with highly molecular mobility were introduced the differentiation into a cardiomyocyte lineage. Therefore, molecularly mobile surfaces were useful as new cell culture environments for the modulation of cellular function by regulating the cytoskeleton-related signaling pathway.

Free Research Field

バイオマテリアル工学