Study of sensitive immunosensor with the ZnO-coated plasmonic chip

2016 Fiscal Year Final Research Report

• Project/Area Number: 25286032
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Nanobioscience
• Research Institution: Kwansei Gakuin University (2016); National Institute of Advanced Industrial Science and Technology (2013-2015)
• Principal Investigator: TAWA Keiko 関西学院大学, 理工学部, 教授 (80344109)
• Co-Investigator(Kenkyū-buntansha): 梅津 光央 東北大学, 工学(系)研究科(研究院), 教授 (70333846)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: プラズモン / 格子 / サンドイッチアッセイ / IL-6 / 蛍光 / 酸化亜鉛

Outline of Final Research Achievements

Interleukin-6（IL-6), a cytokine relating inframmatory and autoimmune activity, was detected with fluorescence assays using a plasmonic chip. In their assays, the way of surface modification, sample volume, incubation time and mixing solution, were found to influence the detection sensitivity. When the assay was revised in the point of a rapid and easy process, the detection sensitivity was not compromised compared to assays with sufficient sample volume and assay
time. In the assay with the ZnO-plasmonic chip and bispecific antibody, the limit of detection was found to be pM level for 10 microL of sample solution and 20 min of assay time.

Free Research Field

ナノバイオサイエンス