Localized surface plasmon sensing for subtype determination of influenza viruses in a specimen with ultra-small volume

2015 Fiscal Year Final Research Report

• Project/Area Number: 25286062
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Optical engineering, Photon science
• Research Institution: Muroran Institute of Technology
• Principal Investigator: KANO Hiroshi 室蘭工業大学, 工学(系)研究科(研究院), 准教授 (80322874)
• Co-Investigator(Kenkyū-buntansha): HASEGAWA Hideki 国立感染症研究所, 感染病理部, 部長 (30301790) ; MORIGAKI Kenichi 神戸大学, 遺伝子実験センター, 准教授 (10358179)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 表面プラズモン / 屈折率測定 / インフルエンザウイルス / サブタイプ判定

Outline of Final Research Achievements

Development of a method for quick subtype determination of influenza viruses is important because infectious and　pathogenic properties of viruses are different. In this research, we proposed a method in which refractive index change due to antigen-antibody reaction between target viruses with certain subtype and monoclonal antibodies fixed in a micro-channel on a sensing substrate. To realize this concept, we successfully improved stability and sensitivity of a measurement system for virus detection, confirmed a measurement principle for faster data acquisition, and developed a technique to modify chemical property of a substrate surface to locate certain antibodies in a controlled region.

Free Research Field

光計測工学