Development of immunoassay for bio-markers using compact disk type microchip

2015 Fiscal Year Final Research Report

• Project/Area Number: 25288065
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: Kyushu University
• Principal Investigator: Imato Toshihiko 九州大学, 工学(系)研究科(研究院), 教授 (50117066)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: フローイムノアッセイ / コンパクトディスク型マイクロチップ / バイオマーカー / 電気化学検出 / 蛍光検出

Outline of Final Research Achievements

Immunoassay is one of the most sensitive and selective analytical　methods,　which　have　used　for　many　analytical　fields　such　as　environmental　monitoring, clinical diagnosis etc. However this method requires somewhat tedious and complicated procedures, such washing, additions of reagent and simple etc. Therefore immunoassay with more simple and less procedure has been expected for rapid immunoassay. In this project, a simple and rapid immunoassay method was developed by using a compact disk-type microchip with an electrochemical detector, a fluorometric detector or a chemiluminescence detector. The most characteristics of the developed method was the flow of the liquids on the reservoirs on the microchip was achieved by using centrifugal force. In addition, the sequence of the flow could be controlled by the rotation speed of the microchip. The analyses of immunoglobulin A, glucose and nonionic surfactant in environmental water have been achieved by the developed method.

Free Research Field

分析化学