Development of the Near-infrared Differential Laser-induced Surface Deformation Microscope and its Application to the Measurements on Rheological Properties of Single Living Cells

2015 Fiscal Year Final Research Report

• Project/Area Number: 25288070
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: Tokyo University of Science
• Principal Investigator: Yui Hiroharu 東京理科大学, 理学部, 教授 (20313017)
• Co-Investigator(Renkei-kenkyūsha): TORIGOE HIDETAKA 東京理科大学, 理学部, 教授 (80227678) ; NAKAMURA TAKESHI 東京理科大学, 生命医科学研究所, 教授 (60362604) ; ASAI KEI 埼玉大学, 理工学研究科, 准教授 (70283934)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 細胞 / 力学応答 / 近赤外光 / 顕微鏡 / 光圧 / パワースペクトル

Outline of Final Research Achievements

We have developed a near-infrared laser-induced surface deformation (NIR-LISD) microscope to measure the rheological properties of single living cells in non-destructive and non-contact manner. The LISD method enabled us to extend the upper limit of the frequency for the dynamic viscoelastic measurements of single cells up to 300 kHz compared to c.a. 1 kHz in the use of the conventional methods such as atomic force microscope and optical stretcher. By the extension of the frequency, it was indicated that the LISD method had the potential to discriminate in viscoelastic properties between normal and cancerous cells. Although we needed to pay attention to the thermal damage of the cells in the irradiation of the visible laser, it was shown that the damage was diminished by the application of the infrared laser. Furthermore, we successfully measured the power spectra of single cells by the reflection-type arrangement of the detection system in the NIR-LISD microscope.

Free Research Field

分析化学