Bio-analytical Application of Fluorescent Probes Incorporated with a New Sensing Mechanism

2015 Fiscal Year Final Research Report

• Project/Area Number: 25288079
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Bio-related chemistry
• Research Institution: Kyushu University
• Principal Investigator: Ojida Akio 九州大学, 薬学研究科(研究院), 教授 (10343328)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 蛍光プローブ / センシング / 金属イオン / バイオイメージング

Outline of Final Research Achievements

This research focused on the bio-analytical application of the fluorescent probes by exploiting the AM-contact (arena-metal ion) emission shift, which is induced by the direct contact between metal ion and fluorophore within their Wan-der Waals radii. In this research we successfully applied a cadmium ion complex to the ratiometric detection of hydrogen sulfide, an bioactive gas, in living cells. We also designed the fluorescent probes appended with a cyclic ligand, which selectively shows a large emission shift upon binding with silver ion. We also successfully applied AM-contat sensing to the ratiomettic detection of zinc ion by the elaborated design of the metal binding unit.

Free Research Field

ケミカルバイオロジー