Three-dimensional measurement of blood cell behavior using digital holographic microscopy and confocal micro-PIV

2016 Fiscal Year Final Research Report

• Project/Area Number: 25289027
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Fluid engineering
• Research Institution: The University of Tokyo
• Principal Investigator: Oishi Masamichi 東京大学, 生産技術研究所, 技術専門職員 (70396901)
• Co-Investigator(Renkei-kenkyūsha): OSHIMA Marie 東京大学, 大学院情報学環, 教授 (40242127) ; FUJII Teruo 東京大学, 生産技術研究所, 教授 (30251474) ; KINOSHITA Haruyuki 東京大学, 生産技術研究所, 特任助教 (40466850)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 赤血球 / デジタルホログラフィ / ３次元計測 / マイクロPIV / 粒子追跡法

Outline of Final Research Achievements

In order to clarify the specific behavior of red blood cells (RBCs) such as deformation, rotational motion of the membrane and axial migration which have a large influence on the blood flow in the capillaries, we developed the "digital holographic microscope (DHM)" and tried to measure the three-dimensional motion of RBCs and its surrounding flow.
Firstly, three-dimensional shape of RBC was measured using high magnification DHM. Then, the DHM-PTV (Particle Tracking Velocimeter) system which can measure the three-dimensional flow field was developed by detecting and tracking tracer particles dispersed in the surrounding flow. This system achieved submicron spatial resolution and particle position detection accuracy.
In addition, in order to improve the reproducibility of RBC measurement, we designed microchannels that can control the position of RBCs passively by flow. As a result of verifying the performance of these channels using DHM, RBCs were controlled within the range of about 5um.

Free Research Field

工学