Development of DNA-based Technology for Accurate and Rapid Assessment of Species Diversity

2015 Fiscal Year Final Research Report

• Project/Area Number: 25289172
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Civil and environmental engineering
• Research Institution: Ehime University
• Principal Investigator: Watanabe Kozo 愛媛大学, 理工学研究科, 准教授 (80634435)
• Co-Investigator(Kenkyū-buntansha): OMURA Tatsuo 東北大学, 未来科学技術共同研究センター, 教授 (30111248) ; KATO Mikio 大阪府立大学, 高等教育推進機構, 教授 (30204499) ; MIYAKE Yo 愛媛大学, 理工学研究科, 准教授 (90345801)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: DNA種分類 / 次世代シークエンシング / 底生動物 / 河川 / 種多様性 / 系統発生

Outline of Final Research Achievements

Using high-throughput Next-generation Sequencing (NGS), we rapidly assessed species richness of stream macroinvertebrate communities among 10 sites in the Shigenobu River catchment in Ehime Prefecture Japan. A conventional morphological taxonomic identification found 64 taxa among 8921 individuals collected from the catchment. Our NGS analysis produced 165,508 reads of mitochondrial COI amplicons. Based on DNA barcoding analysis of the NGS data, 39,337 was hit and 128 species were identified. Further, we found a significant positive correlation between numbers of sequence reads and morphology-based abundance among 33 taxonomic groups (1 class, 3 orders, and 30 families). We also applied DNA taxonomy analysis using PTP model, and the presence of 298 species in the catchment was estimated.

Free Research Field

応用生態工学