Development of an exhaustive detection technique for viable waterborne bacteria by using fluorescence activated cell sorter and next-generation sequencing

2016 Fiscal Year Final Research Report

• Project/Area Number: 25289176
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Civil and environmental engineering
• Research Institution: National Institute of Public Health
• Principal Investigator: Akiba Michihiro 国立保健医療科学院, その他部局等, その他 (00159336)
• Co-Investigator(Kenkyū-buntansha): 岸田 直裕 国立保健医療科学院, その他部局等, その他 (10533359) ; 常田 聡 早稲田大学, 理工学術院, 教授 (30281645)
• Research Collaborator: FUJIMOTO Naoshi 東京農業大学, 応用生物科学部醸造科学科, 教授 (50297595)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 水道 / 微生物 / 遺伝子検査

Outline of Final Research Achievements

In this study, we aimed to develop an exhaustive detection technique for viable waterborne bacteria by using fluorescence activated cell sorter (FACS) and next-generation sequencing (NGS). The developed FACS-NGS method was evaluated using process water samples collected in a drinking water treatment plant, comparing with a conventional cloning and Sanger sequencing (culture-dependent) method. As a result, microbial communities observed in the process water samples were different between the FACS-NGS method and culture-dependent method. Alphaproteobacteria was the major class determined by the culture-dependent method. In contrast, Alphaproteobacteria and Gammaproteobacteria were identified as the two major classes in the FACS-NGS method. The results suggested that the developed FACS-NGS method can detect diverse viable waterborne bacteria.

Free Research Field

水道工学