Qualitative and quantitative control of megakaryocytic differentiation based on comprehensive understanding of hematopoietic cell behaviors

2015 Fiscal Year Final Research Report

• Project/Area Number: 25289295
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Osaka University
• Principal Investigator: Taya Masahito 大阪大学, 基礎工学研究科, 教授 (60144127)
• Co-Investigator(Kenkyū-buntansha): OJIMA Yoshihiro 大阪大学, 大学院基礎工学研究科, 助教 (20546957)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 血小板 / in vitro生産 / 巨核球分化 / 細胞株

Outline of Final Research Achievements

This study was conducted aiming the construction of platelet production in vitro. First, we clarified that both intracellular oxidative stress and polyploidy inducers were important for the promotion of megakaryocytic differentiation. However, platelets generated from CHRF cells did not show the aggregating response due to the malfunction of GP1B protein. Next, the gp1b gene was introduced into CHRF cell and a stable cell line was successfully constructed. Finally, though the mRNA expression of gp1b gene increased 500 times comparing to the original CHRF cells, the expression of GP1B protein could not be confirmed.

Free Research Field

生物化学工学