2016 Fiscal Year Final Research Report
Development of a gene-trap strategy for the transcriptionally silent genes of non-coding RNAs in the target cells
| Project/Area Number |
25290034
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
Ishida Yasumasa 奈良先端科学技術大学院大学, バイオサイエンス研究科, 准教授 (10221756)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Keywords | 遺伝子トラップ / ＵＰＡＴｒａｐ / 非コードＲＮＡ遺伝子 / ＥＳ細胞 / マウス |
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| Outline of Final Research Achievements |
In order to inactivate, by insertional mutagenesis, all protein-coding genes in mouse undifferentiated embryonic-stem (ES) cells, the knockout mouse project (KOMP) had been carried out in the USA, Canada, UK, and Germany as a large-scale and international collaborative effort. In Japan, on the other hand, a gene-trapping strategy termed UPATrap, which we had originally developed, was further upgraded in the national bio-resource project (NBRP) of Monka-sho (MEXT). In the current research, we have tried to establish a novel methodology for the random disruption (trapping) of the transcriptionally silent genes of non-coding RNAs in the target cells.
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| Free Research Field |
マウスの分子遺伝学と免疫学
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