The production of the organ deficient model using rat pluripotent stem cells, and organ regeneration by blastocyst complementation procedure

2015 Fiscal Year Final Research Report

• Project/Area Number: 25290037
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: National Institute for Physiological Sciences
• Principal Investigator: HIRABAYASHI MASUMI 生理学研究所, 行動・代謝分子解析センター, 准教授 (20353435)
• Co-Investigator(Renkei-kenkyūsha): HARA HIROMASA 自治医科大学, 分子病態治療研究センター, 助教 (50751244)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 胚性幹細胞 / 異種キメララット / ノックアウトラット / 胚盤胞補完法 / 臓器欠損 / 人工ヌクレアーゼ / CRISPR/Cas9

Outline of Final Research Achievements

Pancreas- and kidney-deficient rats were prepared by knocking-out (KO) the Pdx1 gene and the Sall1 gene, respectively. By applying blastocyst complementation, both organs could be regenerated from rat ES cells in homozygous KO rat individuals. Knocking-out the Foxn1 gene resulted in mutant rats lacking thymus and T-cell population. Interspecies blastocyst complementation with mouse ES cells gave birth to viable rat offspring carrying mouse thymus. Thus, allogenic or xenogenic stem cells successfully contributed to regeneration of target organs in the KO models.

Free Research Field

発生工学