2015 Fiscal Year Final Research Report
High sensitive detection of single nucleotide mutation of virus genome by chemically modified-peptide nucleic acid
| Project/Area Number |
25290073
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Medical genome science
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Keywords | 遺伝子診断 / 核酸塩基 / ペプチド核酸 |
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| Outline of Final Research Achievements |
Peptide nucleic acid(PNA) is a DNA anaologue which has been known to possesses good sequence specificity. We introduced various types of tolane analogues at the end of PNA and succeeded to improve the sequence specificity. We studied if PNA-tolane can discriminate singel base-mismatched sequence that induces to the drug-resistance against an influenza virus drug. As a result, we found that PNA-tolane could detect the virus genome mutaion in a sequence specific manner.
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| Free Research Field |
ゲノム医科学
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