2015 Fiscal Year Final Research Report
Dynamic regulation of chromatin by histone chaperone
| Project/Area Number |
25291001
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OKUWAKI Mitsuru 筑波大学, グローバル教育院, 准教授 (50322699)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 遺伝子 / ウイルス / ゲノム / 発生・分化 / クロマチン |
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| Outline of Final Research Achievements |
Aim of this study is to reveal the physiological function of histone chaperone TAF-I. TAF-I KO mice showed the embryonic lethality along with severe anemia and immature vascular remodeling until 12.5 day post conception. TAF-I KO ES cells showed the significant growth retardation and the delay of embryonic body formation mimicking the early embryo. From cell culture analyses, we found that TAF-I is involved in transcriptional regulation of interferon-stimulated genes through its histone H1 chaperone activity. From biochemical analyses, we revealed the regulatory mechanism of histone H1 chaperone activity of TAF-I through its intra-molecular binding. We also established the live cell imaging method of adenovirus genome in infected cells using TAF-I as a probe.
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| Free Research Field |
分子生物学
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