Studies of nascent chains that monitors protein localization machineries

2015 Fiscal Year Final Research Report

• Project/Area Number: 25291006
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Molecular biology
• Research Institution: Kyoto Sangyo University
• Principal Investigator: CHIBA Shinobu 京都産業大学, 総合生命科学部, 准教授 (20523517)
• Co-Investigator(Renkei-kenkyūsha): ITO Koreaki 京都産業大学, 研究機構, シニアリサーチフェロー (90027334)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 翻訳アレスト / 翻訳途上鎖 / タンパク質局在化

Outline of Final Research Achievements

To understand the molecular mechanisms of how regulatory arrest peptides, such as Bacillus subtilis MifM or Escherichia coli SecM could regulate their own translation elongation, we studied interactions between nascent polypeptides and the ribosomes. Our genetic, biochemical or structural studies revealed several detail features of interactions between nascent chain and the ribosome in the exit tunnel as well as near the active center of the ribosome. We also studied YidC, a membrane protein insertase that is required for releasing elongation arrest of MifM, and found several biochemical properties of the YidC that are important for its functions.

Free Research Field

分子生物学