Structural analysis of PLN/SERCA regulatome toward understanding of the mechanism of Ca2+-regulation in the cardiac muscle cells

2016 Fiscal Year Final Research Report

• Project/Area Number: 25291012
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: The University of Tokyo
• Principal Investigator: Ogawa Haruo 東京大学, 分子細胞生物学研究所, 准教授 (40292726)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 膜蛋白質 / イオンポンプ蛋白質 / X線結晶解析

Outline of Final Research Achievements

SR calcium uptake is mediated by a SERCA2a, whose activity is reversibly regulated by its direct mediator phospholamban (PLN). PLN is the most important mediator of β adrenergic signal, and it controls SERCA2a depending on its phosphorylation state. Recently many regulators that controls the PLN/SERCA2a complex was discovered. They are thought to form a large-scale complex (PLN/SERCAa regulatome). However, due to lack of efficient expression/purification system for SERCA2/PLN, the details of the mechanisms how they regulate PLN/SERCAa complex is unclear. Thus, we aimed to elucidate the mechanism of regulation of PLN/SERCA2a by regulators with using our own large expression and purification system.

Free Research Field

構造生物学