Molecular and Structural Basis of Nitrogen Removal by Anammox Bacteria

2015 Fiscal Year Final Research Report

• Project/Area Number: 25291015
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Tottori University
• Principal Investigator: Nagano Shingo 鳥取大学, 工学(系)研究科(研究院), 教授 (60286440)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: アナモックス / ヘム / 脱窒 / 水質浄化 / 窒素循環

Outline of Final Research Achievements

Anammox bacteria produce dinitorgen from nitrite and ammonium under anaerobic condition. The key enzyme of nitorogen metabolism of anammox is hydrazine synthase, which produces hydrazine from nitric oxide and anmmonium. In this study, we have prepared 10-L scale membrane bioreactor for cultivation of anammox bacteria. Large amount of HZS was extracted from anammox bacteria and highly purified HZS are obtained by anion exchange and hydroxyapatite chromatography. We have also cloned HZS gene (hzsABC) from anammox bacteria genome and constructed monocistronic and polycistronic expression vector of HZS. Although some of HZS A subunit lost heme c, we have confirmed expression of HZS in E. coli.

Free Research Field

酵素化学