Regulatory mechanisms of cellular functions by integrins and their ligands

2016 Fiscal Year Final Research Report

• Project/Area Number: 25291026
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Osaka University
• Principal Investigator: Sekiguchi Kiyotoshi 大阪大学, たんぱく質研究所, 寄附研究部門教授 (50187845)
• Co-Investigator(Kenkyū-buntansha): 山田 雅司 大阪大学, たんぱく質研究所, 助教 (90304055) ; 二木 杉子 大阪大学, たんぱく質研究所, 助教 (00403014)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 細胞外マトリックス / 細胞接着 / 幹細胞 / 細胞増殖 / ニッチ / リンパ管

Outline of Final Research Achievements

Mice deficient of polydom, a newly identified integrin alpha9beta1 ligand, were generated to study the function of polydom. We found that i) polydom was secreted from mesenchymal cells and deposited around lymphatic vessels; ii) the lymphatic plexus failed to remodel into collecting lymphatic vessels in polydom-deficient mice and thereby died immediately after birth due to severe edema; iii) the expression of Foxc2, a transcription factor involved in lymphatic vessel remodeling, was reduced in polydom-deficient mice; iv) polydom was capable of binding to angiopoietin-2 that has been known to regulate lymphangiogenesis. These results indicate that polydom regulates lymphatic vessel remodeling through binding to angiopoietin-2, thereby promoting the expression of Foxc2.

Free Research Field

生化学・細胞生物学