Roles of syntaxin 17 in the formation and function of the mitochondria-associated ER membrane

2015 Fiscal Year Final Research Report

• Project/Area Number: 25291029
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Tokyo University of Pharmacy and Life Science
• Principal Investigator: TAGAYA MITSUO 東京薬科大学, 生命科学部, 教授 (30179569)
• Co-Investigator(Kenkyū-buntansha): INOUE Hiroki 東京薬科大学, 生命科学部, 講師 (10294448) ; HASHIMOTO Yoshitami 東京薬科大学, 生命科学部, 助教 (50616761) ; ARASAKI Kohei 東京薬科大学, 生命科学部, 講師 (70609990)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 小胞体 / ミトコンドリア / syntaxin17 / レジオネラ / 神経変性疾患

Outline of Final Research Achievements

The MAM（mitochondria-associated membrane）is one of the subdomains of the ER and participates in various cellular phenomena. We found that syntaxin 17 (Syn17) is localized in the MAM and plays the following functions: (1) Syn17 promotes mitochondrial fission by defining the localization and activity of the mitochondrial fission factor Drp1. (2) Syn17 regulates Ca2+ homeostasis of the ER. (3) Syn17 regulates the localization of PGAM5, a protein phosphatase for Drp1. (4) MAP1B-LC1 mediates the link of Syn17 with microtubules and Drp1. (5) Upon infection, Legionella degrades Syn17, and one of its effectors is responsible for this degradation.

Free Research Field

機能生物化学