Clarification of molecular mechanism for the catabolism of glycans on glycoproteins in budding yeast

2016 Fiscal Year Final Research Report

• Project/Area Number: 25291030
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Tadashi Suzuki 国立研究開発法人理化学研究所, 糖鎖代謝学研究チーム, チームリーダー (90345265)
• Co-Investigator(Renkei-kenkyūsha): HARADA Yoichiro 国立研究開発法人理化学研究所, 糖鎖代謝学研究チーム, 特別研究員 (80464147) ; HIRAYAMA Hiroto 国立研究開発法人理化学研究所, 糖鎖代謝学研究チーム, 協力研究員 (50525847)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 糖鎖代謝 / 出芽酵母 / N型糖鎖 / PNGase / オリゴ糖転移酵素

Outline of Final Research Achievements

This study aimed at clarifying the catbaolic mechanism of glycans on glycoproteins in budding yeast. We found that the cytosolic PNGase-indepedent formation of free N-glycans were mediated by oligosaccharyltransferase. We also found lack of evidence for the occurrence of catabolic alpha-glucosidase/beta-mannosidase/beta-hexosaminidase in this yeast. We also identified several endogenous substrate for the cytoplasmic PNGase involved in the catabolism of glycoproteins.

Free Research Field

糖鎖生化学