Nanoquantification of the regulatory mechanism of molecular dynamics by development of super-resolution microscopy using single molecule imaging

2015 Fiscal Year Final Research Report

• Project/Area Number: 25291032
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Biophysics
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: Tokunaga Makio 東京工業大学, 生命理工学研究科, 教授 (00192659)
• Co-Investigator(Renkei-kenkyūsha): SOGAWA KUMIKO (SAKATA KUMIKO) 東京工業大学, 生命理工学研究科, 准教授 (20291073)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: １分子イメージング / バイオイメージング / 細胞機能と構造 / 超解像顕微鏡 / 生体分子計測 / 生細胞分子動態

Outline of Final Research Achievements

We have developed an integrated method for quantitative analysis of molecular dynamics and interactions, combining multiple methods of microscopy, multi-color single-molecule imaging, single molecule tracking and super-resolution microscopy. We have improved resolution in multi-color single-molecule microscopy by optimization of illumination and stabilization of optics. We also imporved accuracy in analysis method for superesolution single-molecule microscopy by optimizing alignment accuracy. The improved integrated microscopy provides a means to obtain new view of spatio-temporal and kinetic parameters of molecular dynamics and interactions in living cells. We carried out imaging analysis of molecules involved in signal transduction and translation.

Free Research Field

生物物理学