Meiotic G2/M-phase transition in response to maturation-inducing hormone

2015 Fiscal Year Final Research Report

• Project/Area Number: 25291043
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Cell biology
• Research Institution: Ochanomizu University
• Principal Investigator: Kishimoto Takeo お茶の水女子大学, サイエンス&エデュケーションセンター, 客員教授 (00124222)
• Co-Investigator(Kenkyū-buntansha): OKUMURA Eiichi 東京工業大学, 大学院生命理工学研究科, 助教 (00323808) ; TACHIBANA Kazunori 東京工業大学, バイオ研究基盤支援総合センター, 准教授 (60212031)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 細胞周期 / ホルモン / 分裂期 / 卵成熟 / Cdk1 / ホルモン受容体 / 細胞内情報伝達 / 閾値

Outline of Final Research Achievements

Immature oocytes arrest at the G2/M-phase border, and the maturation-inducing hormone stimulates release of this arrest via activation of cyclin B-Cdk1, the M-phase governor. Here we investigated the intracellular signal transduction mechanism underlying this release of the cell cycle arrest. We identified molecular components of the binding protein of the maturation-inducing hormone which is located on the oocyte surface. We further revealed the presence of the self-calming system that cancels the stimulus of insufficient levels of maturation-inducing hormone, leading to prevention of an unexpected activation of cyclin B-Cdk1.

Free Research Field

細胞生物学