Comprehensive analyses of the protein factors that interact with transcription factors in plants

2015 Fiscal Year Final Research Report

• Project/Area Number: 25291055
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Saitama University
• Principal Investigator: TAKAGI Masaru 埼玉大学, 理工学研究科, 教授 (40357348)
• Co-Investigator(Kenkyū-buntansha): MITSUDA Nobutaka 産業技術総合研究所, 生物プロセス研究部門, 主任研究員 (80450667)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 転写因子 / シロイヌナズナ / キメラリプレッサー / WD40タンパク質 / タンパク質相互作用 / トランスリプレッション / 転写抑制 / 酵母ツーハイブリッド

Outline of Final Research Achievements

In this project, we performed comprehensive analyses of the WD40 proteins that interact with transcription factors in Arabidopsis, using the trans-repression system and yeast two hybrid system with cDNA library containing only transcription factors, which we had developed. We prepared transgenic Arabidopsis that expressed the chimeric genes of the WD40 protein fused with the SRDX repression domain, and screened the lines with altered phenotype. As a results, we identified that 30 lines that expressed each of WD40-SRDX exhibited altered morphological phenotype. We focused the dwarf lines among them and analysed the transcription factors that interact with the WD40 protein using the yeast two hybrid system.

Free Research Field

植物分子生物学