Identification of the gametocidal gene that induce chromosome breakage in common wheat

2016 Fiscal Year Final Research Report

• Project/Area Number: 25292007
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Science in genetics and breeding
• Research Institution: Kyoto University
• Principal Investigator: Nasuda Shuhei 京都大学, (連合)農学研究科(研究院), 准教授 (10273492)
• Research Collaborator: FRIEBE Bernd ; AKHUNOV Eduard D. ; GILL Bikram S. ; HOUBEN Andreas ; DOLEZEL Jaroslav ; YOSHIOKA Motohiro ; MIZUNO Nobuyuki ; SAKAI Natsuto ; YAMADA Hajime ; MURATA Kazuki
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 配偶子致死 / マッピング

Outline of Final Research Achievements

The objective of this study is to identify the gametocidal gene that induce chromosome breakage in wheat. We took the approach of map-based cloning to identify the gene. First, we roughly mapped the gene to the tip of the long arm of chromosme T4B-4Ssh. Then, by adopting molecular markers of the syntenic chromosomal region of rice, we could narrow down the chromosomal region harboring the gametocidal gene to a 15 cM interval between two markers. It may give an impression that the gene is still distant from the molecular markers, but eventually the region is physically estimated to be 1.3 Mb because of inflation of apparent recombination near the gene in the population tested here. Our estimation is that if we could identify the markers as close as 1.0 cM, the gene would be within 100 kb from the markers. The results of the present study will promote identification and cloinig of the causal gene.

Free Research Field

植物遺伝学