Metabolic regulation in dormant buds of deciduous fruit tree-Study of function of low temperature signal

2017 Fiscal Year Final Research Report

• Project/Area Number: 25292019
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Horticultural science
• Research Institution: University of Tsukuba
• Principal Investigator: SUGAYA Sumiko 筑波大学, 生命環境系, 教授 (90302372)
• Co-Investigator(Kenkyū-buntansha): 瀬古澤 由彦 筑波大学, 生命環境系, 助教 (90361310)
• Project Period (FY): 2013-04-01 – 2018-03-31
• Keywords: 休眠 / ニホンナシ / メタボローム解析

Outline of Final Research Achievements

To investigate mechanisms of occurance of necrosis and abnormal flowering of dormant buds in deciduous fruit tree, changes in mebobolome including a metabolome analysis, phytohormone analysis, bud burst and dormancy related gene expression analysis were performed using Japanese pear under different temperature conditions. From the results of these analysis, temperature fluctuation (TF) during both endodormancy and ecodormancy periods affected on many pathways of metabolism including sugar, organic acids, amino acids. Especially, pentose phosphate pathway was affected by TFs. In addition cyanamide treatment affected on beta-alanine, proline, glucose-6-phosphate. These results indicated that supply of energy source and oxidative stress would be factors invloved in dormancy breaking. Gene expresions of DAM, gene expresions related phytohormone synthesis and metabolism were chaned by TF treatments.

Free Research Field

果樹園芸学