2016 Fiscal Year Final Research Report
Identification of genes controlling astringency in Chinese-type PCNA (pollination constant non-astringent) and its application to breeding for PCNA-type persimmon
Project/Area Number |
25292020
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Horticultural science
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Research Institution | Ryukoku University (2016) Kyoto University (2013-2015) |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
神崎 真哉 近畿大学, 農学部, 准教授 (20330243)
佐藤 明彦 国立研究開発法人農業・食品産業技術総合研究機構, 果樹研究所, 上席研究員 (30355440)
山根 久代 京都大学, (連合)農学研究科(研究院), 講師 (80335306)
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Project Period (FY) |
2013-04-01 – 2017-03-31
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Keywords | 園芸学 / 果樹 / 甘渋性 / タンニン |
Outline of Final Research Achievements |
The accuracy of AFLP markers linked to the locus of natural astringency-loss in Chinese-type PCNA persimmon were confirmed and then, the markers were used as probes for fosmid/BAC library constructed from Diospyros lotus, which is a close diploid relative of D. kaki, for constructing contig to identify the locus of natural astringency-loss. However, the results showed that the fosmid/BAC library is hard to use directly for identifying the locus probably due to the difference of genome structure between D. kaki and D. lotus. On the other hand, RNA-seq analysis using segregated progeny between Chinese-type PCNA and non-PCNA indicated that the genes related to transportation in the cell were differently expressed among them, as well as the genes related to flavonoid biosynthesis pathway.
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Free Research Field |
果樹園芸学
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