Molecular Analysis for heterostyly by using genomics and transcriptmics approches

2016 Fiscal Year Final Research Report

• Project/Area Number: 25292023
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Horticultural science
• Research Institution: Okayama University
• Principal Investigator: Ushijima Koichiro 岡山大学, 大学院環境生命科学研究科, 准教授 (20379720)
• Co-Investigator(Kenkyū-buntansha): 鈴木 孝征 中部大学, 公私立大学の部局等, 講師 (50535797) ; 赤木 剛士 京都大学, (連合)農学研究科(研究院), 助教 (50611919) ; 池田 和生 山形大学, 農学部, 准教授 (80555269)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 異形花型自家不和合性

Outline of Final Research Achievements

Heterostyly is one of self-incompatibility that is a genetic system to reject the self-pollen and be linked to floral morph polymorphism．In this study, to elucidate the molecular mechanism of Linum heterostyly, we isolated the candidate gene controlling heterostyly and characterized the S locus region. Four candidate gene were newly isolated by transcriptome analysis. Genome analysis revealed the S haplotype specific region at least reached more that 1 Mbp. All candidate genes were included in this region but the relative locations were unclear. We further shotgun sequenced the genome of Linum grandiflorum and de novo assembly. The assembled contigs will give new insights on the study of heterostyly.

Free Research Field

植物分子遺伝学